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Titre: | Insecticidal activity and physiopathological effects of Cotula cinerea crude extract against Culex pipiens |
Auteur(s): | Demouche, Lahna Acheuk, F. Mokrane, K. Abdellaoui, K. Lakhdari, W. Bensouici, C. Dehliz, A. |
Mots-clés: | Cotula cinerea Culex pipiens Methanolic extract Insecticidal activity Physiopathological effects |
Date de publication: | 2023 |
Collection/Numéro: | Tropical Biomedicine / Vol. 40, N°2 (2023);pp. 241-249 |
Résumé: | The development of new alternatives strategies to synthetic insecticides aimed at reducing pest
populations by developing pesticides based on plant extracts without negative effects in non target
organisms and environment. The present study was undertaken in order to assess the insecticidal activity
of the crude methanolic extract of the Algerian Asteraceae Cotula cinerea, against the larval and the
pupal stage of Culex pipiens (Diptera: Culicidae). It is also to determine the chemical composition of
the used extract, and to understand the mechanism of toxic action of the tested extract. Based on the
preliminary tests, five concentrations of the crude methanolic extract of C. cinerea (0.62, 1.25, 2.50, 3.75,
and 5 mg/mL) were tested for their insecticidal activity according to the protocol recommended by the
World Health Organization. The chemical profile of the extract was also obtained by high performance
liquid chromatography (HPLC). Histopathological effects and inhibition of acetylcholinesterase activity
in treated mosquitoes with LC90 were examined to elucidate the mechanism of the toxic effect of the
tested extract (48 h post treatment). Eight compounds have been identified by HPLC. That includes four
flavonoids (rutin, quercetin, myrcetin and cathechin), three phenolic acids (benzoic acid, vanillic acid,
p-coumaric acid) and one alkaloid (berberine). C. cinerea methanolic extract showed good larvicidal and
pupicidal activities with LC50 and LC90 values of 1.10 and 4.37 mg/mL respectively against pupae, 24h
post treatment and 1.26, 2.35 mg/mL respectively against the fourth instar larvae. Data of enzymatic
assay performed on LC50 and LC90 pupae and larvae revealed prominent neurotoxic effects. C. cinerea
extract reduced the activity of acetylcholinesterase (AChE) enzyme in a concentration dependent
manner. Obtained inhibition percentages, 48 h after treatment, were 35.11 ± 7.44 and 51.83 ± 4.04% for
pupal stage and 30.98 ± 2.97 % and 48.77 ± 4.72% for the fourth instar larvae for LC50 and LC90 values
respectively. Treated larvae and pupae showed also histopathological damages in the pupal cuticle
and larval midgut. The results of this study showed that C. cinerea crude methanolic extract could be
considered as an eco-friendly alternative for mosquito contro |
URI/URL: | https://doi.org/10.47665/tb.40.2.016 http://dlibrary.univ-boumerdes.dz:8080/handle/123456789/13308 |
Collection(s) : | Publications Internationales
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