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Titre: Effect of exhaustion of olive pomace on cellulases production from Trichoderma reesei RUT C30 and identification of the enzyme complexes
Auteur(s): Boutiche, Malika
Bennacer, Amel
Aissaoui, Mohammed
Meziant, Leila
Saci, Fairouz
Siar, El-Hocine
Boulaouad, Ilyes
Bekrar, Amel
Fiala, Samah
Hemoui, Hadjer
Sahir-Halouane, Fatma
Mots-clés: Cellulases
Exhaustion
Olive pomace
SDS-PAGE
Trichoderma reesei
Date de publication: 2024
Editeur: Springer
Collection/Numéro: Vegetos (2024);
Résumé: Olive industry generates large quantities of solid by-products such as olive pomace. The objective of present work is to study the effect of exhaustion (residual oil extraction) by acetone solvent on the improvement of cellulase production using olive pomace as substrate, and to identify the crude enzymatic extract by electrophoretic analysis. The production of cellulases was done by the solid fermentation process using Trichoderma reesei RUT C30 as cellulolytic fungus grown on olive pomace. Cellulolytic activity was assessed by the filter paper method (FPase). Electrophoretic analysis by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) was performed for the identification of enzymatic extract of T. reesei RUT C30. Chemical composition (lipids, proteins, sugars and ash) was significantly (P < 0.05) affected after olive pomace exhaustion. In general, exhaustion by acetone solvent increased the fiber fraction. Exhaustion improved the results of FPase activity compared to those obtained on untreated substrate, giving values of 2.63 IU/gds and 1.1 IU/gds, respectively. The results of SDS-PAGE analysis revealed that the crude enzymatic extract contains two different enzyme complexes including cellulases and xylanases.
URI/URL: https://link.springer.com/article/10.1007/s42535-024-00974-5
https://doi.org/10.1007/s42535-024-00974-5
http://dlibrary.univ-boumerdes.dz:8080/handle/123456789/14629
ISSN: 0970-4078
Collection(s) :Publications Internationales

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